Pectin Lyase and Pectate Lyase from Debaryomyces nepalensis Isolated from Apple
نویسندگان
چکیده
منابع مشابه
An unusual pectate lyase from a Bacillus sp. with high activity on pectin: cloning and characterization.
The gene pelA encoding a pectate lyase from the strain Bacillus sp. BP-23 was cloned and expressed in Escherichia coli. The nucleotide sequence of a 1214 bp DNA fragment containing pelA gene was determined, revealing an ORF of 666 nucleotides that encoded a protein of 23233 Da. The deduced amino acid sequence of the encoded enzyme showed homology to pectate lyases A, B, C and D from Fusarium so...
متن کاملEnzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of double bonds produced an increase in light...
متن کاملRegulation of the Aspergillus nidulans Pectate Lyase
Aspergillus nidulans pectate lyase was purified from culture filtrates. The enzyme catalyzed a random eliminative cleavage reaction, had an apparent molecular weight of 40,000, and a pl of 4.2. Pectate lyase antisera were produced and used to identify pectate lyase clones in a cDNA expression library. Thirteen of 14 clones identified immunologically cross-hybridized. The identity of the single-...
متن کاملPectate lyase C from Bacillus subtilis: a novel endo-cleaving enzyme with activity on highly methylated pectin.
The gene yvpA from Bacillus subtilis was cloned and expressed in Escherichia coli. It encoded a pectate lyase of 221 amino acids that was denominated PelC. The heterologously expressed enzyme was purified by His-tag affinity chromatography and characterized. PelC depolymerized polygalacturonate and pectins of methyl esterification degree from 22 % to 89 %, exhibiting maximum activity on 22 % es...
متن کاملAspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield
BACKGROUND Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked in cell walls and are not easily accessible to enzymes and chemicals. RESULTS We sh...
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ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2006
ISSN: 1816-4935
DOI: 10.3923/jm.2006.152.159